Figure 1.

Demonstration of stomatal counting algorithm. A, Reflective intensity layer of an OT measurement of the abaxial epidermis of a sorghum leaf section. B, OT measurement from (A) overlaid with automatic detection of stomata (red). Automatically detected stomata in close proximity are highlighted in green. C, Association between the median SD of samples where stomates have been both manually and automatically counted. Four to six FOV were used to calculate the median values for each genotype by manual and automatic methods. A linear model regressing automatic counts on manual counts is fitted (red) and the standard error of the model is shown (gray). The associated P-value significance threshold and r² value of the model are inset.