FIG 2.

SplintQuant for quantification of cmRNA relative to the total mRNA. (A) Schematic of SplintQuant method mechanism. (B) Circularization efficiency was evaluated by M13 primer sets for all transcripts and cmRNA (MaSp1 and FSLP), respectively. MaSp1 backbone, the PCR product of common area of cDNA obtained from MaSp1 linear mRNA and MaSp1 cmRNA. MaSp1 BSJ, the PCR product of BSJ sequence of cDNA obtained from MaSp1 cmRNA. FSLP backbone, the PCR product of common area of cDNA obtained from FSLP linear mRNA and FSLP cmRNA. FSLP BSJ, the PCR product of cDNA obtained from FSLP cmRNA. (C) The circularization efficiency of the MaSp1 and FSLP transcripts was determined by RT-qPCR.