TABLE 2.
Oligonucleotides used for this study
| Primer | Sequence | Note |
|---|---|---|
| cirMaSp1 BK-F | CCAGGTCTCACACCACCACCACCACCAC | pcirMaSp1 construction |
| cirMaSp1 BK-R | CCAGGTCTCAGGTAGCATTATGTTCAGATAAGGTCGTTAATCT | |
| cirMaSp1-F | CCAGGTCTCATACCAGCGGTCGCGGCGGTCTGGG | |
| cirMaSp1-R | CCAGGTCTCAGGTGAGTACCCTGAGAACCCAGGCCGC | |
| cirFSLP-F | TACCGGGGGAGCAGGCGGATCAGG | pcirFSLP construction |
| cirFSLP-R | GGTGGTAGCCACCCGGACCGCTGC | |
| MaSp1 linear donor | TTGCAGCCGCAGCCTGGCCGTCGTTTTAC | Hybridization with non-BSJ sequence of MaSp1 transcripts |
| MaSp1 linear acceptor | CAGGAAACAGCTATGACCCAGCACCGCCCA | |
| MaSp1 circular donor | AACGCAGATCCATCTGGCCGTCGTTTTAC | Hybridization with BSJ sequence of MaSp1 cmRNA |
| MaSp1 circular acceptor | CAGGAAACAGCTATGACCGACCGCTGGTAG | |
| FSLP linear donor | GCGCCGTCGATCGCTGGCCGTCGTTTTAC | Hybridization with non-BSJ sequence of FSLP transcripts |
| FSLP linear acceptor | CAGGAAACAGCTATGACGATCGGACCATCT | |
| FSLP circular donor | AACGCAGATCCATCTGGCCGTCGTTTTAC | Hybridization with BSJ sequence of FSLP cmRNA |
| FSLP circular acceptor | CAGGAAACAGCTATGACGCTCCCCCGGTAG | |
| M13-F | GTAAAACGACGGCCAG | Amplification of ligated products |
| M13-R | CAGGAAACAGCTATGAC |