TABLE 3.
Strains and plasmids used in this study
| Strains and plasmids | Description | Source |
|---|---|---|
| Strains | ||
| Diaphorobacter sp. strain JS3051 | 2,3-dichloronitrobenzene utilizer | 9 |
| E. coli strains DH5α | supE44 lacU169 (ϕ80dlacZ ΔM15) recA1 endA1 hsdR17 thi-1 gyrA96 relA1 | Novagen |
| E. coli BL21(DE3) | F- ompT hsdSB(rB- mB-) gal dcm lacY1 (DE3) | Novagen |
| Plasmids | ||
| pET28-28a(+) | IPTG inducible expression vector, Kanr | Novagen |
| pETDuet-1 | IPTG inducible coexpression vector, Ampr | Novagen |
| pET-dccA | dccA fragment inserted into pET-28a(+) between NdeI and BamHI; Kanar | 13 |
| pET-dccAB | dccAB fragment inserted into pET-28a(+) between NdeI and BamHI; Kanar | 13 |
| pETDuet-DCB | NcoI-SacI fragment containing dcbAaAb and NdeI-KpnI fragment containing dcbAcAd inserted into pETDuet-1; Ampr | 13 |