TABLE 4.
Oligonucleotide primers used for site-directed mutagenesis
| Oligonucleotides | Sequences(5′–3′) | Description |
|---|---|---|
| DCBE204D-F | AACTTCGTTGGTGACGATTACCACGTTGGTTGGAC | pETDuet-DCB204 |
| DCBE204D-R | GTCCAACCAACGTGGTAATCGTCACCAACGAAGTT | |
| DCBE204I-F | GGTCCAACCAACGTGGTATATGTCACCAACGAAGTTTTCA | pETDuet-DCB204 |
| DCBE204I-R | TGAAAACTTCGTTGGTGACATATACCACGTTGGTTGGACC | |
| DCBN258V-F | GGGACTACTACGCTGGTGTCTTCTCTGCTGACATGG | pETDuet-DCB258 |
| DCBN258V-R | CCATGTCAGCAGAGAAGACACCAGCGTAGTAGTCCC | |
| DCBL293H-F | GCTCGTATCTACCGTTCTCATCTGAACGGTACTGTTTTCC | pETDuet-DCB293 |
| DCBL293H-R | GGAAAACAGTACCGTTCAGATGAGAACGGTAGATACGAGC | |
| DCBI350F-F | TGACGCTGTTCAGCGTTCTTTCGGTCCGGC | pETDuet-DCB350 |
| DCBI350F-R | GCCGGACCGAAAGAACGCTGAACAGCGTCA |