Fig. 2. AptNCL post-insertion and pH sensitivity of AptNCL-LippH and PEGylated AptNCL-PEGLippH formulations. (A) Schematic representation of post-insertion of cholesterol-conjugated AptNCL on the surface of LippH and PEGLippH. (B) and (C) Characterization of liposomes before and after AptNCL post insertion on the surface of LippH and PEGLippH (B) Agarose gel electrophoresis. Lanes: a, AptNCL- PEGLippH; b, Free PEGLipPH; c, free AptNCL (C) the change of hydrodynamic diameter and the polydispersity index of LippH, AptNCL-Lip, PEGLippH, and AptNCL-PEGLippH (D) calcein dye releasing assay from AptNCL-LippH and PEGylated AptNCL-PEGLippH formulations. (mean ± SD, n = 3).
