Fig. 3. Flow cytometry analyses for the uptake of free AptNCL,PEGLippH, and AptNCL-PEGLippH in cultured cells; for each cell line (A) MDA-MB-231, (B) MCF7 (C) A549, (D) fibroblasts; (upper part of A, B, C, D) show the mean fluorescence intensity (MFI) measurements after treatment with free AptNCL,PEGLippH, and AptNCL-PEGLippH. (Lower part of A, B, C, D) show flow cytometry histogram analysis after treatment with free AptNCL,PEGLippH, and AptNCL-PEGLippH. Cells were treated with free AptNCL (200 nM), or PEGLippH (120 mM), or AptNCL-PEGLipPh (200 nM AptNCL/120 mM PEGLippH) and compared to untreated cells. AptNCL labeled with Alexa647 and PEGLippH labeled with rhodamine. (mean ± SD, n = 3).
