Figure 1.

Schematic diagram of the full-length SVA genome and Generation of re-SVA-EGFP infectious clone. (A) The construction strategy of SVA clone. Four separate fragments, A to D, were assembled into the pBluescript II SK (+) vector using homologous recombination and the unique restriction enzyme sites. A hammerhead ribozyme and a HDV ribozyme was located at the 5 'and 3' ends of the SVA genome, respectively. The full-length genome is under the control of a CMV promoter. (B) Immunofluorescent assay for detecting SVA infection and EGFP expression. The SVA infected was labeled in red, EGFP was in green, and DAPI stained cell nucleus in blue. (C) One-step growth curve of re-SVA-EGFP infectious clone.