Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 8;9:840655. doi: 10.3389/fvets.2022.840655

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Jia, Sun, Tang, Zhang, Wang, Cai and Meng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

pANTXR1 interference reduced SVA replication in PAM-Tang cells. The SVA at an MOI of 0.1 was added to the PAM-Tang cells after siRNA interference. (A) The qPCR analysis of relative pANTXR1 mRNA levels changes in PAM-Tang cells (**p < 0.01). (B) The Western blot assay for the expression of SVA VP2 and VP0 proteins. (C) The qPCR analysis of SVA copy numbers in the supernatant (***p < 0.001). (D) The TCID₅₀ assay for SVA titers in supernatants post siRNA1 interference (*p < 0.05). (E) The TCID₅₀ assay for SVA titers in pANTXR1 overexpressed PAM-Tang cells at 6 hpi (*p < 0.05). (F) The western blot assay for the expression of SVA VP2, VP0 proteins and pANTXR1 from pANTXR1 overexpressed PAM-Tang cells at 6 hpi. (G) Immunofluorescent assay for pANTXR1 (in red) and EGFP (in green) expression level in the pANTXR1 overexpressed PAM-Tang cells, and the colocalization of pANTXR1 with EGFP was indicated by arrow. Bars, 200 μm.