Figure 4.

Exogenous re-expression of the pANTXR1 promoted SVA infectivity. pCAGGS-pANTXR1 plasmid was transfected into Hela KO cells and PAM-Tang KO cells, and the pANTXR1 re-expressing cells were inoculated with SVA at an MOI of 0.1 (A) The qPCR analysis of SVA copy numbers in the Hela KO cells (***p < 0.001). (B) The TCID₅₀ assay for SVA titers in Hela KO cells (***p < 0.001). (C) The western blot assay for the expression of SVA VP2 and VP0 proteins and pANTXR1 from Hela KO cells. (D) The qPCR analysis of SVA copy numbers in the PAM-Tang KO cells (***p < 0.001). (E) The TCID₅₀ assay for SVA titers in PAM-Tang KO cells (***p < 0.001). (F) The western blot assay for the expression of SVA VP2 and VP0 proteins and pANTXR1 from PAM-Tang KO cells. (G) Immunofluorescent assay for pANTXR1 (in red) and EGFP (in green) expression level in the pANTXR1 re-expressed PAM-Tang KO cells and Hela KO cells. The colocalization of pANTXR1 with EGFP was indicated by arrow. Bars, 200 μm.