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. 2022 Apr 8;9:840655. doi: 10.3389/fvets.2022.840655

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Copyright © 2022 Jia, Sun, Tang, Zhang, Wang, Cai and Meng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SVA internalization depends on membrane cholesterol. PAM-Tang cells were pretreated with inhibitors prior to SVA infection. (A) The Western blot analysis for the expression of SVA VP2 and VP0 proteins from PAM-Tang cells that were pretreated with inhibitors. (B) The cell viability test assay. (C) The TCID₅₀ assay for viral titers (**P < 0.01, ***P < 0.001). (D) Immunofluorescent assay for EGFP (in green) expression level in the cells pretreated with MβCD. Bars, 200 μm.