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Copies (cp) of mutations measured when RNA containing the mutation was diluted into no, low, or high background of WT gRNA. Low background is 100 copies/well, and high background is 10,000 copies/well, where “copies” refers to copies of genomes of WT gRNA. Markers show the average across three replicate wells, and error bars represent standard deviations. In some cases, the error bar is not visible because it is smaller than the marker. rxn, reactions equivalent to one well.
