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. 2022 Apr 11;7(2):e00164-22. doi: 10.1128/msystems.00164-22

FIG 1.

FIG 1

The cytotoxicity of tRF mimics is significantly stronger than that of tRNA halves on HCT-8 cells. (A) Typical UHPLC chromatogram of 5′- and 3′-tRNA halves of tRNA-Leu(CAG) under UV at 260 nm. (B) Urea-polyacrylamide gel electrophoresis of 5′- and 3′-tRNA halves of tRNA-Leu(CAG) confirming that the purified tRNA was successfully digested into two products. (C) UHPLC-MS analysis of 5′- and 3′-tRNA halves of tRNA-Leu(CAG) showing that the molecular weights of the digestion products were in accordance with the 5′- and 3′-tRNA halves of E. coli tRNA. (D) Cytotoxic comparison of tRNA halves, tRF mimics, and individual tRNAs of tRNA-Val(UAC) on HCT-8 cells. (E) Cytotoxic comparison of tRNA halves, tRF mimics, and individual tRNAs of tRNA-Leu(CAG) on HCT-8 cells. Data are shown as the means ± SD from three independent experiments. ****, P < 0.0001 (by one-way ANOVA followed by post hoc analysis).