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. 2022 Jan 4;106(4):802–813. doi: 10.1093/biolre/ioab240

Figure 6.

Figure 6

Mean ± SEM expression of PDYN in the arcuate nucleus of POS-CYC (n = 7), NEG-NONCYC (n = 8), and NEG-CYC cows (n = 6). Panel A shows quantification by qPCR, being relative to the geometric mean of housekeeping genes. Panels B (number of cells) and C (expression/cell silver grains/cell—gpc) show results from ISH. *P < 0.05. qPCR analysis showed that expression of PDYN was marginally higher (P < 0.05) in POS cows than in NEG-CYC cows, but this was not confirmed by ISH.