Table 3.

Mean (± SEM) GNRH1 and Kiss1 expression in the POA and GnRH and Kiss peptide levels in the ME of POS-CYC, NEG-CYC, and NEG-NONCYC cows (n = 8/group). Levels of expression are given relative to the housekeeping genes, as indicated in Materials and methods

Group	POS-CYC	NEG-CYC	NEG-NONCYC
POA GNRH1	5.73 ± 0.67	6.47 ± 0.94	6.01 ± 1.29
POA Kiss1	25.69 ± 5.06	22.06 ± 3.34	22.69 ± 3.34
ME GnRH peptide (ng/mg)	1.14 ± 0.10	1.05 ± 0.14	0.90 ± 0.13
ME Kiss peptide (ng/mg)	0.037 ± 0.037	0.044 ± 0.026	0.035 ± 0.042