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. 2022 Apr 11;13(2):e03849-21. doi: 10.1128/mbio.03849-21

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Copyright © 2022 Santos et al.

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FIG 7 — Involvement of HA-EHD1 in multivesicular body formation. (A) Representative anti-HA antibody and anti-APSK antiserum (top) or anti-Vps26 antiserum (bottom) double-staining IFA images of trophozoites that expressed HA-EHD1 trophozoites after 1, 3, and 24 h of induction by tetracycline. Bar = 10 μm. (B) Representative immunoelectron image of a trophozoite expressing HA-EHD1 24 h after tetracycline induction, stained with 15-nm gold–anti-HA. c, cytosol; MVB, multivesicular body; ILV, intraluminal vesicle. Bar = 200 nm.