TABLE 2.
The sequences of PNA oligomers and their in vitro cell-free transcription/translation inhibition activity measured using S. aureus ribosomesa
| PNA oligomers according to E. coli numberingb | Sequence (5′→3′) | IC50 (μM) | Slope |
|---|---|---|---|
| 1862-10 (1889-10) | GAAGCTAACC | 20.6 ± 8.7 | 0.3 ± 0.03 |
| 1862-12 (1889-12) | CAGAAGCTAACC | 2.2 ± 0.9 | 0.5 ± 0.07 |
| 1862-14 (1889-14) | CGCAGAAGCTAACC | 1.3 ± 0.7 | 0.5 ± 0.09 |
| 1873-10 (1900-10) | GTAGCTTCGC | 6.3 ± 2.4 | 0.6 ± 0.10 |
| 1873-12 (1900-12) | TCGTAGCTTCGC | 4.9 ± 1.2 | 0.7 ± 0.06 |
| 1873-14 (1900-14) | ATTCGTAGCTTCGC | 3.8 ± 0.5 | 0.7 ± 0.06 |
a
Errors reported are standard errors of duplicate experiments.
b
S. aureus numbering is given in parentheses.