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Remote conditioning by rhythmic compression reduced cAMP content and PKA activity in heart of AMI rats. (A) Levels of cAMP in the heart of AMI rat were determined by ELISA, N = 6. (B,C) Levels of phosphorylated CREB (p-CREB) and total CREB in heart were detected by western blot. The corresponding quantification data of p-CREB to CREB ratio are shown in right panel. All values represent as means ± SD. N = 6. ****p < 0.0001 vs. CON.
