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. 2022 Mar 14;13(2):e03739-21. doi: 10.1128/mbio.03739-21

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Copyright © 2022 Li et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 6 — Adaptation of rCH/SX/2016-S1_HNXP+S3_HNXP to Vero cells. (A) Schematic illustration of the construction of rCH/SX/2016-S_HNXP, rCH/SX/2016-S1_HNXP, rCH/SX/2016-S2_HNXP, rCH/SX/2016-S1_HNXP+S3_HNXP, and rCH/SX/2016-S1_HNXP+S4_HNXP. S:1-1387aa, S1:1-729aa, S2:730-1387aa, S1_HNXP+S3_HNXP:1-976aa, S1_HNXP+S4_HNXP:1-729aa and 977-1387aa. (B) Vero cells were infected with rCH/SX/2016-S_HNXP, rCH/SX/2016-S1_HNXP, rCH/SX/2016-S2_HNXP, rCH/SX/2016-S1_HNXP+S3_HNXP, and rCH/SX/2016-S1_HNXP+S4_HNXP. Cells were harvested at 24 and 48 hpi. Then RT-qPCR was performed to detect the relative expression of mRNA of PEDV N protein (C). Vero cells were infected with rCH/SX/2016-S_HNXP and rCH/SX/2016-S1_HNXP+S3_HNXP. Infected cells were fixed at 36 h postinfection and immunolabeled with Fluorescein (FITC)-AffiniPure Goat Anti-Mouse IgG (H+L). Nuclei were labeled with DAPI (blue).