FIG 2.
ZCholO and ZCholI robustly colonize the small intestine but do not cause death. (A) Kaplan-Meier plot of P3 infant mice orally infected with a lethal dose of 107 CFU of the indicated strains; n = 6 per group. Median time to moribundity was 31 hours postinfection for infant mice infected with the toxigenic parent strain. Differences in the survival curves were assessed with a log-rank (Mantel Cox) test. (B) V. cholerae burden in the small intestine (SI) of challenged mice at time of moribundity (ZTox) or at 48 hours postinfection (ZChol strains). (C) CFU per SI for P5 infant mice orally infected with a nonlethal 105 CFU of indicated strains and sacrificed at 20 hours postinoculation. Differences in CFU burdens were assessed with a Mann-Whitney U test. Horizontal bars indicate geometric means of each group. ns, not significant, * p < 0.05.