FIG 3.

ZChol^O and ZChol^I provide a similar gauge of vaccine-mediated protection as ZTox without causing diarrheal disease. (A) Schematic overview of GF vaccination regimen and experimental protocol. (B) P5 pups born to germfree female mice orally immunized with either a live (n = 5 GF dams) or killed oral cholera vaccine (OCV) (n = 5 GF dams) were orally inoculated with ZTox or ZChol^O or ZChol^I. CFU per SI in these pups were determined at 20 hours post inoculation (10⁵ CFU per infant mouse inoculum) with the indicated strains. Asterisks denote mice with visible diarrhea. Differences in CFU burdens were determined by a Mann-Whitney U test. Horizontal bars indicate geometric means of each group.