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Isothermal titration calorimetry study of the binding of different ligands to the ligand-binding domain of chemoreceptor PctD. (Upper panel) Raw data for the titration of 15 to 22 μM protein with 9.6-μL aliquots of 0.5 to 10 mM ligand solutions. (Lower panel) Concentration-normalized and dilution heat-corrected raw data for the titration with acetylcholine and choline. The continuous line is the best fit with the “one binding site model” of the MicroCal version of ORIGIN.
