TABLE 2.
Modified glycosylation of A(H3N2) IAV reduces HA binding by rhSP-D variantsa
| rhSP-D | Hemagglutinin-inhibiting concn (μg/mL) for: |
|||
|---|---|---|---|---|
| Turkey red blood cells |
Guinea pig red blood cells |
|||
| NGI-1-treated virus | Untreated virus | NGI-1-treated virus | Untreated virus | |
| Monomer | >10 | 1.25 | 5 (partial inhibition) | 1.25 |
| Dodecamer | >10 | 1.25 | 2.5 | 0.63 |
| Truncated/ D325A/R343V | >10 | 1.25 | 10 | 5.0 |
| Truncated | >10 | 10 (partial inhibition) | >10 | >10 |
a
Binding of the rhSP-D variants to glycome-modified HA from A(H3N2) IAV grown with or without 10.0 μM NGI-1 was tested in two independent HI tests with different lots of red blood cells. rhSP-D binding to virus HA was determined by measuring the concentration required to inhibit 4 HAU of NGI-1-treated or untreated viruses. Data from one representative experiment run in triplicate are shown.