FIG 1.

Minimap2 provides improved error, sensitivity, and runtime for host filtering over the current open-source pipeline. Comparison of aligners for host filtering on 1 million CAMI-Sim simulated reads by error (a) and human reads (b) failed to align to the reference (false-negative rate). (c and d) Time (c) and processing rate (d) comparison across aligners of 1 million, 10 million, and 50 million CAMI-Sim simulated reads. Minimap2 is shown for 100 million and 250 million reads. (e) False-negative rate of host filtering on data with real reads combined from separate exome sequencing and nonhuman metagenomics studies.