FIG 1.

Genetic map of the mid locus within esx1 of the donor strain mc²155. The mid region of the donor strain M. smegmatis mc²155 was defined by linkage mapping to Msmeg0069–0078 (8). Conserved core esx genes, homologs of which are typically found in esx loci, are shown above the locus. The encoded core and other Esx proteins are highly conserved (>96.6% amino acid identity), in contrast to the Mid proteins; percentages below the encoding mid genes indicate amino acid sequence identities between donor (mc²155) and recipient (MKD8) orthologs. Msmeg0071 is poorly conserved at its N terminus (27%), but its C terminus is highly conserved (93% amino acid identity).