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mid confers self-identity and determines mating compatibility. (A) Map of esx1 and mid indicating the relevant genes used in the study. (B) Mating pairs boxed inside the shaded rectangle generated transconjugants at high frequencies (>1 × 10−4 per donor) (DCT “+”). Unboxed mating pairs produced no or very few transconjugants (<1 × 10−9 donors). In all crosses, parent donor and recipient counts were similar.
