FIG 3.

Spike proteins of variant-driven viral entry into HeLa cells expressing human, mouse, marmoset, or koala ACE2 orthologs. Cell entry of the virions pseudotyped with the WT, Kappa, Delta, or B.1.618 spike proteins or individual mutations of these variants’ spike proteins was tested on HeLa cells expressing human, mouse, marmoset, or koala ACE2 orthologs. Luciferase activity was determined after 2 days of infection, and data were normalized to the WT (D614G) of the individual experiment. All infections were performed in triplicate, and the data are representative of three independent experiments (mean ± SD). n.s., no significance; *, P < 0.05; **, P < 0.01; ***, P < 0.001. Significance assessed by one-way ANOVA.