The newly discovered M159 protein from MYXV-Tol belongs to the C7 superfamily of host range proteins. (A) Representation of the genome organization of MYXV-Tol (GenBank accession no. MK836424). Orange shows the disrupted M009L gene. Blue and purple ORFs show the new gene “cassette” identified and the location of the M157, M158, M159, and M160 genes in MYXV-Tol. Ub, ubiquitin; Pol, polymerase. (B) Phylogenetic analysis of C7-like members from 23 different poxviruses. EctV, Ectromelia virus; CaPV, Capripox virus; TePV, Taterapox virus; MPV, Monkeypox virus; VarV, Variola virus; VacV, Vaccinia virus; CoPV, Cowpox virus; NYPV, NY_014 poxvirus; RaPV, Raccoonpox virus; SKPV, Skunkpox virus; VPV, Volepox virus; CotPV, Cotia virus; MuPV, Murmansk poxvirus; YKV, Yokapox virus; BePV, BeAn 58058 virus; YMTV, Yaba monkey tumor virus; SqPV, Squirrelpox virus; SwPV, Swinepox virus; DPV, Deerpox virus; LSDV, Lumpy skin disease virus; SPV, Sheeppox virus; GPV, Goatpox virus; EpPV, Eptesipox virus; TPV, Turkeypox virus; FIPV, Flamingopox virus; PPV, Pigeonpox virus; PePV, Penguinpox virus.