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YQL proteins from Actinobacteria hydrolyze simple N7-alkylguanosine lesions and interstrand cross-links. (A and B) Denaturing PAGE of reaction products of E. coli YcaQ (Eco) and YQL proteins from Thermomonospora curvata (Tcu) and Thermobifida fusca (Tfu) with 7mG-DNA (A) and NM8-ICL (B) after 5 min and 1 h. Lane 1 of each gel is a no-enzyme control.
