Fig. 15.

Image Acquisition Validation of the Incubot. A) Representative image of live HDF cells using dual white LED illumination (reflected) or single white LED illumination (oblique) with processes uniquely identified using oblique illumination highlighted with (*) (scale bars = 500 µm). B) Uniformity of fluorescent imaging assessed using a Thorlabs green fluorescent slide (FSK2) in greyscale (scale bar = 400 µm) with mean pixel intensity value from each column along the image’s width. C) Live HeLa-GFP visualization using oblique illumination, blue LED fluorescent illumination (acquisition exposure time < 1 sec), and overlay (scale bars = 500 µm). D) Assessment of phototoxicity revealed no significant difference in cell density between LED-exposed and control cell after 24 h of repeat illumination of live HeLa-GFP cells (scale bars = 500 µm, representative images subjected to background subtraction processing in ImageJ). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)