Table 2.

Effects of power-on time and ambient temperature on the output values from the colorimeter and the calculated absorbance (Mean ± standard deviation, n = 5).

		Output value		Absorbance
		I0 (Blank)	I (0.25 mM)	A = −log(I/I0)
Power-on time (at 25 °C)	0 h	405.2 ± 8.0	133.8 ± 4.9	0.481 ± 0.0081
	0.5 h	403.2 ± 8.2	132.6 ± 5.2	0.483 ± 0.0085
	2.0 h	404.0 ± 9.2	132.6 ± 5.2	0.484 ± 0.0073
Temperature	4 °C	371.4 ± 7.2	121.2 ± 4.6	0.487 ± 0.0081
	40 °C	421.2 ± 8.3	139.2 ± 5.3	0.481 ± 0.0078