Skip to main content
. Author manuscript; available in PMC: 2022 Apr 26.
Published in final edited form as: Methods Mol Biol. 2021;2153:307–328. doi: 10.1007/978-1-0716-0644-5_22

Figure 4. BIR kinetics assay using CHEF gel electrophoresis.

Figure 4.

(From data in Elango et al [9] published under open access and a Creative Commons Attribution 4.0 International License). A. The BIR repair kinetics in wild type (SRS2) strain isogenic to AM 1003 (left) and BIR-defective srs2Δ derivative. The aliquots were taken from the liquid cultures undergoing BIR repair before (0h) and following the addition of galactose to the media at indicated time points. The genomic DNA was separated by CHEF. The upper panels represent ethidium bromide stained gels of the separated chromosomes. The middle panels represent the results of Southern blot analysis using an ADE3-specific probe that highlights the donor chromosome III. The bottom panels represent the results of Southern blot analysis using an ADE1-specific probe highlighting the recipient chromosome III. Chr.: chromosome; Trunc. Chr III: truncated chromosome III. B. Quantification of BIR product (top) and of donor chromosome entering the gel (bottom) in wild type (SRS2) and srs2Δ strains from the results of analysis shown in A.