Fig. 2. Comparison of the cytotoxic activity of 1 and 2: the dose–response curve of both compounds (after 48 h treatment) shows similar IC50 values but a stronger effect of 2 at higher concentrations (A). (B) Results of the recovery experiment (treatment followed by 48 h recovery) show more rapid effect on cell viability in 2. (C) Membrane damage, as assessed by lactate dehydrogenase (LDH) leakage, shows the more potent effect of 2. (D) Accumulation of 1 and 2 in the cell as assessed using HPLC-HRMS shows the faster entry of 2 in the cell.
