Table 1.
Genes Encoding for Putative Virulence Factors in Sporothrix globosa
| Putative Function | S. globosa Genomic Region | Best Hit | Similarity (%) | Coverage (%) | Ortholog in S. schenckii | Ortholog in S. brasiliensis |
|---|---|---|---|---|---|---|
| Dimorphism | ||||||
| Hyphal G cyclin 1 | Sequence ID: LVYW01000001.1 Range: 1,025,425 to 1,026,504 |
A. fumigatus G1/S-specific cyclin Cln1s | 67 | 87 | Yes | Yes |
| Cell wall synthesis | ||||||
| Chitin synthase | Sequence ID: LVYW01000005.1 Range: 1,866,742 to 1,869,543 |
A. fumigatus chitin synthase A | 73 | 95 | Yes | Yes |
| Sequence ID: LVYW01000004.1 Range: 3,601,706 to 3,604,024 |
A. fumigatus chitin synthase B | 83 | 75 | Yes | Yes | |
| Sequence ID: LVYW01000001.1 Range: 1,933,042 to 1,935,999 |
A. fumigatus chitin synthase F | 75 | 91 | Yes | Yes | |
| Sequence ID: LVYW01000004.1 Range: 659,665 to 662,220 |
A. fumigatus chitin synthase G | 77 | 97 | Yes | Yes | |
| β-1,3-Glucanosyltransferase | Sequence ID: LVYW01000002.1 Range: 622,419 to 627,869 |
A. fumigatus 1,3-β-glucan synthase catalytic subunit FksP | 85 | 94 | Yes | Yes |
| Dolichol phosphate synthase | Sequence ID: LVYW01000004.1 Range: 4,120,034 to 4,120,861 |
A. fumigatus dolichol phosphate mannosyl-transferase | 77 | 100 | Yes | Yes |
| Protein mannosyltransferase | Sequence ID: LVYW01000002.1 Range: 3,642,281 to 3,643,684 |
A. fumigatus protein O-mannosyl-transferase | 82 | 93 | Yes | Yes |
| Sequence ID: LVYW01000006.1 Range: 1,823,756 to 1,826,095 |
A. fumigatus protein mannosyl-transferase Pmt1 | 71 | 93 | Yes | Yes | |
| α-1,2-Mannosyltransferase | Sequence ID: LVYW01000005.1 Range: 2,631,677 to 2,632,711 |
A. fumigatus α-1,2-mannosyl-transferase (Kre2) | 81 | 80 | Yes | Yes |
| Sequence ID: LVYW01000001.1 Range: 1,590,849 to 1,591,889 |
A. fumigatus α-1,2-mannosyltransferase (Ktr4) | 77 | 87 | Yes | Yes | |
| Sequence ID: LVYW01000003.1 Range: 48,707 to 49,552 |
A. fumigatus α-1,2-mannosyl-transferase (Kre5) | 86 | 100 | Yes | Yes | |
| α-1,6-Mannosyltransferase | Sequence ID: LVYW01000002.1 Range: 3,966,407 to 3,967,531 |
A. fumigatus glycosyl-transferase Och1 | 65 | 82 | Yes | Yes |
| Sequence ID: LVYW01000005.1 Range: 2,421,530 to 2,422,483 |
A. fumigatus α1,6-mannosyl-transferase Mnn9 | 80 | 80 | Yes | Yes | |
| Thermotolerance | ||||||
| Suppresses thermotolerance and modulates capsule formation | Sequence ID: LVYW01000005.1 Range: 1,893,011 to 1,894,318 |
A. fumigatus cAMP-dependent protein kinase-like Sch9 | 86 | 99 | Yes | Yes |
|
Heat shock transcription factor |
Sequence ID: LVYW01000002.1 Range: 2,640,249 to 2,642,180 |
A. fumigatus heat shock transcription factor Hsf1 | 49 | 65 | Yes | Yes |
|
Heat shock-induced protein |
Sequence ID: LVYW01000005.1 Range: 1,292,334 to 1,294,616 |
A. fumigatus heat shock protein/chaperonin Hsp78 | 84 | 92 | Yes | Yes |
|
Invasin, member of Hsp70 |
Sequence ID: LVYW01000002.1 Range: 3,289,290 to 3,291,185 |
A. fumigatus molecular chaperone Hsp70 | 94 | 90 | Yes | Yes |
| Sequence ID: LVYW01000003.1 Range: 4,342,973 to 4,344,541 |
A. fumigatus Hsp70 chaperone BiP/Kar2 | 91 | 99 | Yes | Yes | |
| Sequence ID: LVYW01000003.1 Range: 1,590,876 to 1,592,192 |
A. fumigatus Hsp70 chaperone HscA | 88 | 99 | Yes | Yes | |
| Sequence ID: LVYW01000003.1 Range: 1,425,780 to 1,427,675 |
A. fumigatus mitochondrial Hsp70 chaperone (Ssc70) | 92 | 91 | Yes | Yes | |
| Sequence ID: LVYW01000001.1 Range: 3,575,451 to 3,577,520 |
A. fumigatus Hsp70 chaperone Hsp88 | 72 | 88 | Yes | Yes | |
| Sequence ID: LVYW01000006.1 Range: 1,235,654 to 1,237,159 |
A. fumigatus Hsp70 chaperone BiP | 68 | 91 | Yes | Yes | |
| Sequence ID: LVYW01000003.1 Range: 5,249,506 to 5,251,209 |
A. fumigatus Hsp70 family chaperone Lhs1/Orp150 | 70 | 71 | Yes | Yes | |
| Heat shock protein, mitochondrial chaperone (Hsp60) | Sequence ID: LVYW01000002.1 Range: 857,724 to 859,505 |
A. fumigatus antigenic mitochondrial protein Hsp60 | 87 | 96 | Yes | Yes |
| Heat shock protein involved in thermotolerance (Hsp90) | Sequence ID: LVYW01000005.1 Range: 880,214 to 881,443 |
A. fumigatus molecular chaperone and allergen Mod-E/Hsp90/Hsp1 | 94 | 87 | Yes | Yes |
| Adhesins | ||||||
| Cell wall integrity, adherence, and biofilm formation | Sequence ID: LVYW01000001.1 Range: 1,884,061 to 1,884,846 |
A. fumigatus cell wall glucanase Scw11 | 59 | 41 | Yes | Yes |
| Sequence ID: LVYW01000007.1 Range: 2,857,516 to 2,858,574 |
A. fumigatus cell wall glucanase | 61 | 95 | Yes | Yes | |
| Cell wall morphology, pH-dependent expression (Phr1) | Sequence ID: LVYW01000004.1 Range: 4,497,069 to 4,498,358 |
A. fumigatus β-1,3-glucanosyl-transferase | 69 | 78 | Yes | Yes |
| Sequence ID: LVYW01000001.1 Range: 86,737 to 87,795 |
A. fumigatus β-1,3-glucanosyl-transferase Gel1 | 76 | 78 | Yes | Yes | |
| Sequence ID: LVYW01000003.1 Range: 3,681,474 to 3,682,763 |
A. fumigatus β-1,3-glucanosyl-transferase Gel2 | 69 | 88 | Yes | Yes | |
| Biofilm formation | ||||||
| Transcription Factor (Ndt80) | Sequence ID: LVYW01000002.1 Range: 4,774,368 to 4,775,234 |
A. fumigatus NDT80/PhoG like DNA-binding family protein | 53 | 48 | Yes | Yes |
| Secreted hydrolases | ||||||
| Secreted aspartyl protease (Sap2) | Sequence ID: LVYW01000006.1 Range: 613,730 to 615,028 |
A. fumigatus aspartic-type endopeptidase OpsB | 69 | 88 | Yes | Yes |
| Sequence ID: LVYW01000006.1 Range: 2,678,211 to 2,679,401 |
C. albicans Sap8 | 47 | 81 | Yes | Yes | |
| Sequence ID: LVYW01000001.1 Range: 4,068,222 to 4,069,364 |
A. fumigatus aspartic endopeptidase Pep2 | 81 | 90 | Yes | Yes | |
| Sequence ID: LVYW01000005.1 Range: 1,785,032 to 1,786,228 |
A. fumigatus aspartic endopeptidase Ap1 | 63 | 88 | Yes | Yes | |
| Sequence ID: LVYW01000002.1 Range: 2,154,952 to 2,156,013 |
A. fumigatus aspartic-type endopeptidase CtsD | 57 | 75 | Yes | Yes | |
| Sequence ID: LVYW01000004.1 Range: 2,345,538 to 2,346,500 |
A. fumigatus extracellular aspartic endopeptidase | 66 | 77 | Yes | Yes | |
| Phospholipase | Sequence ID: LVYW01000001.1 Range: 4,901,960 to 4,903,879 |
A. fumigatus lysophospholipase Plb1 | 67 | 96 | Yes | Yes |
| Sequence ID: LVYW01000001.1 Range: 5,943,020 to 5,945,185 |
A. fumigatus phospholipase A2 PlaA | 63 | 87 | Yes | Yes | |
| Lipase | Sequence ID: LVYW01000002.1 Range: 2,894,403 to 2,895,689 |
A. fumigatus secretory lipase | 61 | 89 | Yes | Yes |
| Urease | Sequence ID: LVYW01000001.1 Range: 6,005,160 to 6,007,553 |
A. fumigatus Urease Ure | 84 | 87 | Yes | Yes |
| Melanin production | ||||||
| Polyketide synthase | Sequence ID: LVYW01000001.1 Range: 5,766,340 to 5,772,381 |
A. fumigatus conidial pigment polyketide synthase PksP/Alb1 | 60 | 94 | Yes | Yes |
| Phenoloxidase | Sequence ID: LVYW01000004.1 Range: 3,463,454 to 3,465,208 |
A. fumigatus ferrooxidoreduc-tase Fet3 | 67 | 96 | Yes | Yes |
| Sequence ID: LVYW01000001.1 Range: 5,252,734 to 5,254,242 |
A. fumigatus extracellular dihydrogeodin oxidase/laccase | 48 | 88 | Yes | Yes | |
| Sequence ID: LVYW01000003.1 Range: 3,378,591 to 3,380,357 |
A. fumigatus laccase TilA | 60 | 94 | Yes | Yes | |
| Nutrient uptake | ||||||
| GTPase | Sequence ID: LVYW01000001.1 Range: 2,496,580 to 2,497,089 |
A. fumigatus Rheb small monomeric GTPase RhbA | 89 | 100 | Yes | Yes |
| Sequence ID: LVYW01000006.1 Range: 992,857 to 993,159 |
A. fumigatus RAS small monomeric GTPase Rsr1 | 86 | 72 | Yes | Yes | |
|
Ornithine monooxygenase |
Sequence ID: LVYW01000002.1 Range: 2,281,754 to 2,283,208 |
A. fumigatus L-ornithine N5-oxygenase SidA | 59 | 90 | Yes | Yes |
| Zinc homeostasis | Sequence ID: LVYW01000005.1 Range: 1,316,107 to 1,317,288 |
A. fumigatus high-affinity zinc ion transporter | 67 | 99 | Yes | Yes |
| Sequence ID: LVYW01000005.1 Range: 1,316,173 to 1,317,216 |
A. fumigatus plasma membrane low-affinity zinc ion transporter | 55 | 86 | Yes | Yes | |
| Sequence ID: LVYW01000002.1 Range 1: 1,172,630 to 1,173,124 |
A. fumigatus ZIP family zinc transporter | 83 | 71 | Yes | Yes | |
| Acetyl-CoA synthetase | Sequence ID: LVYW01000002.1 Range: 1,643,562 to 1,644,794 |
A. fumigatus acetyl-coenzyme A synthetase FacA | 88 | 92 | Yes | Yes |
| Serine/threonine kinase | Sequence ID: LVYW01000003.1 Range: 3,006,373 to 3,007,410 |
A. fumigatus carbon catabolite derepressing protein kinase Snf1 | 83 | 69 | Yes | Yes |
| Phosphoenolpyruvate carboxykinase | Sequence ID: LVYW01000003.1 Range 1: 1,604,482 to 1,605,645 |
A. fumigatus pyruvate kinase | 84 | 99 | Yes | Yes |
| Hexose kinase | Sequence ID: LVYW01000001.1 Range 1: 1,447,659 to 1,448,525 |
A. fumigatus hexokinase Kxk | 84 | 95 | Yes | Yes |
| Sequence ID: LVYW01000002.1 Range 1: 4,445,090 to 4,447,084 |
A. fumigatus hexokinase | 64 | 68 | Yes | Yes | |
| Sequence ID: LVYW01000005.1 Range 1: 443,537 to 444,583 |
A. fumigatus glucokinase | 68 | 82 | Yes | Yes | |
| Isocitrate lyase | Sequence ID: LVYW01000006.1 Range: 3,093,278 to 3,094,873 |
A. fumigatus isocitrate lyase AcuD | 83 | 98 | Yes | Yes |
| Sequence ID: LVYW01000005.1 Range: 2,780,931 to 2,782,532 |
C. albicans Isocitrate lyase 1 | 51 | 97 | Yes | Yes | |
| Malate synthase | Sequence ID: LVYW01000002.1 Range 1: 3,545,499 to 3,546,767 |
A. fumigatus malate synthase AcuE | 91 | 99 | Yes | Yes |
| Response to stress | ||||||
| Superoxide dismutase | Sequence ID: LVYW01000002.1 Range: 3,012,655 to 3,013,167 |
A. fumigatus Cu/Zn superoxide dismutase SOD1 | 78 | 97 | Yes | Yes |
| Sequence ID: LVYW01000003.1 Range: 2,047,916 to 2,048,404 |
A. fumigatus cytosolic Cu/Zn superoxide dismutase C. albicans SOD5 | 57 | 71 | Yes | Yes | |
| Catalase | Sequence ID: LVYW01000002.1 Range: 4,168,516 to 4,169,937 |
C. albicans catalase A | 74 | 97 | Yes | Yes |
| Sequence ID: LVYW01000004.1 Range: 3,095,593 to 3,097,812 |
A. fumigatus spore-specific catalase CatA | 71 | 97 | Yes | Yes | |
| Flavohemoglobin-related protein | Sequence ID: LVYW01000005.1 Range 1: 3,049,448 to 3,050,809 |
A. fumigatus flavohemoprotein | 56 | 98 | Yes | Yes |
| Serine/threonine phosphatase | Sequence ID: LVYW01000003.1 Range: 4,774,748 to 4,776,373 |
A. fumigatus calcineurin catalytic subunit CnaA | 85 | 96 | Yes | Yes |
| Thioredoxin | Sequence ID: LVYW01000001.1 Range: 5,596,040 to 5,597,059 |
A. fumigatus thioredoxin reductase Trr1/Trr2 | 77 | 82 | Yes | Yes |
| Sequence ID: LVYW01000001.1 Range: 1,734,081 to 1,734,710 |
A. fumigatus thioredoxin | 61 | 96 | Yes | Yes | |
| Toxins and secondary metabolites | ||||||
| Non-ribosomal peptide | Sequence ID: LVYW01000006.1 Range: 3,263,839 to 3,277,668 |
A. fumigatus nonribosomal siderophore peptide synthase SidC | 45 | 93 | Yes | Yes |
| C6 finger domain protein | Sequence ID: LVYW01000004.1 Range: 4,538,250 to 4,539,548 |
A. fumigatus C6 finger domain protein | 50 | 80 | Yes | Yes |