Evaluation of intra-day and inter-day accuracy and precision of the proposed method in case of different serum samples^a.

Serum samples	Standard method	Proposed method
		Intra-day accuracy and precision (n = 3)				Inter-day accuracy and precision (n = 3)
	Average	Average reading	±CL	RE (%)	RSD (%)	Average reading	±CL	RE (%)	RSD (%)
Patient (1)	10	10.2	±0.0135	2.00	0.98	10.5	±0.0133	5.00	0.95
Patient (2)	23	22.9	±0.0090	0.43	0.44	22.1	±0.0092	3.91	0.45
Patient (3)	27	27.1	±0.0083	0.37	0.37	28	±0.0081	3.70	0.36
Patient (4)	15	15.5	±0.0109	3.33	0.65	14.5	±0.0113	3.33	0.69
Patient (5)	18	18.3	±0.0101	1.67	0.55	18	±0.0101	0.00	0.56
Patient (6)	99	98.8	±0.0043	0.20	1.00	100	±0.0043	1.01	1.00
Patient (7)	78	78.0	±0.0049	0.00	1.30	79	±0.0048	1.28	1.31
Patient (8)	130	130.6	±0.0038	0.46	0.80	129	±0.0038	0.77	0.80
Patient (9)	210	210.2	±0.0030	0.10	0.50	212	±0.0030	0.95	0.50
Patient (10)	105	105.4	±0.0042	0.38	0.90	103	±0.0042	1.90	1.00
Patient (11)	222	221.7	±0.0029	0.14	0.50	225	±0.0043	1.96	0.10
Patient (12)	102	102.3	±0.0043	0.29	0.10	100	±0.0029	1.35	0.40

^aRE: percent relative error, RSD (%): relative standard deviation and CL: confidence limits were calculated from: CL = ±tS/√n (the tabulated value of t is 4.303, at the 95% confidence level; S = standard deviation and n = number of measurements).