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. 2022 Apr 12;13:836573. doi: 10.3389/fphar.2022.836573

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Copyright © 2022 Milder, Zybura, Cummins and Marrs.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Behavioral profile of zebrafish larvae exposed to PTZ. The following graphs displays the average large count (y-axis) of larvae. (A) The average larval large count is depicted per 15 min interval (x-axis) of the tracking session. Zebrafish larvae were tested PTZ concentrations at 5 mM, 10 mM, 20 mM, and 40 mM (n = 70 fish/solution/concentration). Embryo medium (EM) was used as the control. Significance indicated by *p < 0.05 PTZ vs. EM, two-way ANOVA, Tukey’s post-hoc. (B) The total large count (y-axis) over 90 min is displayed. The respective treatment groups (x-axis). Analyses (one-way ANOVA, Tukey’s post-hoc) are marked * (p < 0.05) to indicate significant differences to embryo medium (EM).