Fig. 8. Polydisperse nanobubbles functionalized with Cy3-labeled cell-penetrating peptide (CPP) and FITC-labeled epidermal growth factor receptor-targeted small interfering RNA (siEGFR), fabricated using the lipid film hydration agitation method for gene therapy of triple negative breast cancer. (A) Nanobubbles fluorescing red, indicating effective loading of the CPPs in the bubble shell. (B) The same nanobubbles fluorescing green, indicating effective loading of the siEGFR in the bubble shell.¹¹².