FIG. 4.

Further controlled evaluation of specific inhibition of AS-1 and AS-7, at a final concentration of 10 μM, on CVB3 structural protein VP1 synthesis. HeLa cells were transfected with 10 μM AS-1, AS-1-s, AS-1-r, AS-7, AS-7-s, or AS-7-r by a Lipofectin method and then infected with CVB3 at an MOI of 0.01. After incubation, the cell-free supernatants were collected, and the viral protein VP1 for each sample was detected (A) and quantitated by densitometry (B). The means ± SD of the controls were normalized to a value of 100. Mean ± SD values of ODN treatments were calculated with respect to the control values. The data from four independent experiments were analyzed. The difference between each control and its respective ODN was statistically significant (P < 0.01).