Fig. 7. Antibacterial testing of the non-quaternized PDMAEMA-EDMA25 and quaternized PDMAEMA-EDMA25Q nanogels. Time-kill curve assay was carried out to estimate the effect of the nanogels on both S. aureus (a) and A. baumannii (c) counts after 1 and 4 h of the treatment at room temperature. The data indicate mean values ± SD. Asterisks represent a significant difference (***p < 0.001) when compared to non-treated control. (b and d) Representative photographs of corresponding agar plates with S. aureus ((b), 5 mg ml−1, representative suspension dilution of 10−5) and A. baumannii ((d), 5 mg ml−1, representative suspension dilution of 10−4) colonies including non-treated controls (NT).
