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| Step | ||
|---|---|---|
| 1 | Conditioning | 3.0 mL MeOH and then 3.0 mL H2O |
| 2 | Loading | 4.0 mL sample |
| 3 | Washing | 3.0 mL H2O |
| 4 | Elution | 2 mL mixture of MeOH/acetic acid (9 : 1, v/v) |
| 5 | Evaporation to dryness with N2 (30 °C) | |
| 6 | Reconstitution with 500 μL of H2O | |
| 7 | Filtration with 0.45 μm filter membrane. CE–CL measurement | |
