Skip to main content
. 2022 Apr 19;2022:7177889. doi: 10.1155/2022/7177889

Figure 4.

Figure 4

LGR6 was reduced in DKD and reversed by MaR1 treatment. (a) Representative immunohistochemistry images showing the localization and expression of LGR6 in kidney of DKD patients. Bar: 100 μm. (b) Representative western blotting analysis images showing the protein levels of LGR6 in DKD mouse model. (c) Representative immunofluorescence images showing the localization and expression of LGR6 in kidney of mouse model. Bar: 100 μm. (d) Representative western blotting analysis images showing the protein levels of LGR6 in HK-2 cells stimulated by high glucose (40 nM). (e) Representative immunohistochemistry images showing the localization and expression of LGR6 in kidney of DKD mouse model with MaR1 intervention. Bar: 100 μm. (f) qRT-PCR showing the mRNA levels of LGR6 in DKD mouse model with MaR1 intervention. (g) Representative western blotting analysis images showing the protein levels of LGR6 in DKD mouse model with MaR1 intervention. (h) qRT-PCR showing the mRNA levels of LGR6 in high glucose-stimulated HK-2 cells with MaR1 intervention. (i) Representative western blotting analysis images showing the protein levels of LGR6 in high glucose-stimulated HK-2 cells with MaR1 intervention. All results are representative of three independent experiments. Data were expressed as mean ± SD; ∗P <0.05; ∗∗P <0.01; ∗∗∗P <0.001.