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. 2021 Dec 28;102(5):545–553. doi: 10.1038/s41374-021-00714-2

Fig. 7. Multispectral imaging and image processing: composite color images and spectral unmixing in NSCLC ADC FFPE tissue stained with p40/TTF-1 duplex IHC and H&E.

Fig. 7

A Color composite image formed from spectrally unmixed monochrome transmitted light images recorded at 510 nm, where eosin primarily absorbs light, and 599 nm, where hematoxylin absorbs light (monochrome images not shown). Monochrome camera images of transmitted light at769 nm where Cy7 CDC primarily absorbs light, staining p40 (B), and 880 nm, where ir870 CDC absorbs light, staining TTF-1 (C). Spectrally unmixed images of p40 (E) and TTF-1 (F). D Two-color composite image formed from the spectrally unmixed hematoxylin (not shown) and TTF-1 (F) images. Images were recorded using a ×20 objective.