Figure 2.

Participant infectivity, proportion of mosquitoes infected, and oocyst density in direct membrane feeding assays

(A) Participant infectivity. Error bars are 95% CIs. p values from generalised linear models (family: binary) testing differences within treatment groups with baseline as reference are shown. The denominator for the proportion of infectious participants is the total number of participants still enrolled at a given timepoint, rather than the number tested at that time point for infectivity; infectivity assays were discontinued when a participant did not infect mosquitoes at two subsequent timepoints and were thereafter considered non-infectious. Full mosquito infection data including the proportion of infectious participants with denominator as total participants tested is shown in appendix 5 (p 6). (B) Mosquito infection rate. Each line represents one participant. Statistical analyses are shown in appendix 5 (p 6). (C) Oocyst density. Box plots show the median (central line), IQR (box limits), upper and lower quartiles plus 1·5 × IQR (whiskers), and outliers for mean oocyst densities in infected mosquitoes within each participant. Wilcoxon sign rank tests for differences in average oocyst density are shown. NC=not calculable.