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. 2022 Apr 14;185(8):1308–1324.e23. doi: 10.1016/j.cell.2022.02.022

Figure 3.

Figure 3

In vitro reconstituted oskar RNP condensates are selectively permeable

(A) Scheme of the in vitro condensate ageing assay. Single confocal slices and representative line profiles (dotted arrows) shown.

(B) 4 nm-thick tomographic slices of condensates at 0 and 30 min after addition of 400 nM atto633-oskar 3′UTR. Black arrowheads, naked RNA strands.

(C) Condensates assembled with oskar 3′UTR-atto633 (blue) and EGFP-tagged Bruno or Hrp48 (green). mRFP-PTB (red) or TMR (tetramethyl rhodamine)-BSA (red) was added after 30 min. Single confocal slices shown; partition coefficient of mRFP-PTB calculated from 10 fields of view.

(D) Tomographic slices (4 nm thick) of 2 μM mRFP-PTB added to 30 min aged Bruno-oskar 3′UTR condensates. White arrowheads, protein clusters. See also Figure S4; Video S3.