| REAGENT/RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-Oskar (1:3000; WB & IF) | In-house | N/A |
| Rabbit anti-Hrp48 (1:2000; WB) | In-house | N/A |
| Rabbit anti-Bruno (1:1000; WB & 1:3000 IF) | In-house | N/A |
| Rabbit anti-PTB (1:2000; WB) | In-house | N/A |
| Rabbit anti-EGFP (1:5000; WB) | Torrey Pines Biolabs | Cat# TP401; RRID:AB_10890443 |
| Rabbit anti-Histone H3 (1:2500; WB) | Abcam | Cat# ab1791; RRID:AB_302613 |
| Rat anti-Vasa (1:500; IF) | In-house | N/A |
| Mouse anti-Even-skipped (1:500; IF) | Gift of Justin Crocker | ∗DSHB 2B8 |
| Chemicals | ||
| 1,6-Hexanediol | Sigma | Cat# 240117 |
| Colchicine | Sigma | Cat# C9754 |
| Insulin | Sigma | Cat# I9278 |
| Fetal Bovine Serum (FBS) | Life Technologies | Cat# 10082147 |
| Schneider’s Drosophila medium | Life Technologies | Cat# 21720024 |
| SumoStar protease | Life Sensors | Cat# 4110 |
| PEG-4000 | Thermo Scientific | Cat# EL0011 |
| ATP | Thermo Scientific | Cat# R0441 |
| RNase A | Thermo Scientific | Cat# EN0531 |
| Benzonase | Sigma | Cat# E1014 |
| Tetramethyl Rhodamine BSA | Thermo Scientific | Cat# A23016 |
| Shandon ImmuMount | Thermo Scientific | Cat# 9990402 |
| Critical commercial assays | ||
| Effectene Transfection reagent | Qiagen | Cat# 301425 |
| X-tremeGENE HP DNA Transfection reagent | Roche | Cat# 06 366 236 001 |
| MEGAscript T7 transcription kit | Thermo Scientific | Cat# AMB13345 |
| StrepTrap HP | Merck | Cat# GE28-9075-47 |
| HisTrap HP | Merck | Cat# GE17-5248-01 |
| HiLoad 16/600 Superdex 200pg | Merck | Cat# GE28-9893-35 |
| Plasmids | ||
| pFastBAC 1-mRFP-PTB | This study | N/A |
| pCoofy63-BrunoFL-EGFP | This study | N/A |
| pCoofy63-BrunoΔN -EGFP | This study | N/A |
| pCoofy63-Hrp48FL-EGFP | This study | N/A |
| pCoofy63-Hrp48ΔC -EGFP | This study | N/A |
| UASp-attB-K10-Bruno FL-EGFP | This study | N/A |
| UASp-attB-K10-Bruno ΔN-EGFP | This study | N/A |
| pU6-BbsI-chiRNA | Gratz et al. (2013) | Addgene Cat# 45946 |
| pHD-scarless dsRED | Kate O'Connor-Giles | Addgene Cat# 64703 |
| UASp-attB-K10-EGFP-Hrp48FL | This study | N/A |
| UASp-attB-K10-EGFP-Hrp48ΔC | This study | N/A |
| ∗pHsp83-NLS-HA-tdMCP-2xEGFP | This study | N/A |
| Dendra2-FUS WT | Patel et al. (2015) | N/A |
| ∗pHsp83-NLS-HA-tdMCP-EGFP-FUS LC | This study | N/A |
| MBP-FUS FL-12E | Monahan et al. (2017) | Addgene Cat# 98652 |
| ∗pHsp83-NLS-HA-tdMCP-EGFP-FUS LC 12E | This study | N/A |
| pActin5C-Bruno FL-EGFP | This study | N/A |
| pActin5C-Bruno ΔN -EGFP | This study | N/A |
| Oligonucleotide probes smFISH probes are listed in supplementary figure legends S2 (oskar) and S5 (gurken, bicoid) | Gáspár et al., (2017b) | N/A |
| Fly strains (D. melanogaster) | ||
| w1118 | ∗BDSC | BDSC Cat# 3605; RRID:BDSC_3605 |
| oskar6xMS2/CyO | Lin et al. (2008) | N/A |
| oskar6xMS2:MCP-EGFP/Tm3Sb | Lin et al. (2008) | N/A |
| mRFP-Nup107 | Hampoelz et al. (2019) | N/A |
| w[1118]; PBac{y[+mDint2] GFP[E.3xP3]=vas-Cas9}VK00027 | BDSC | BDSC Cat# 51324; RRID:BDSC_51324 |
| y[1] M{vas-int.Dm}ZH-2A w[∗]; PBac{y[+]-attP-3B}VK00033 | BDSC | BDSC Cat# 24871; RRID:BDSC_24871 |
| vas-phi-ZH2A, PBac{y[+]-attP-9A}VK00018 | BDSC | BDSC Cat# 9736; RRID:BDSC_9736 |
| aretCRISPR-dsRED/CyO | This study | N/A |
| If/CyO; UASp-BrunoFL-EGFP/Tm3ser | This study | N/A |
| If/CyO; UASp-BrunoΔN-EGFP/Tm3ser | This study | N/A |
| bruno-RNAi : P{TRiP.HMC02374}attP2 | BDSC | BDSC Cat# 44483; RRID:BDSC_51324 |
| hrp48-RNAi : P{TRiP.JF01478}attP2 | BDSC | BDSC Cat# 31685; RRID:BDSC_51324 |
| ptb-RNAi : P{TRiP.GLV21034}attP2 | BDSC | BDSC Cat# 35669; RRID:BDSC_51324 |
| If/CyO; UASp- EGFP-Hrp48FL- /Tm3ser | This study | N/A |
| If/CyO; UASp- EGFP-Hrp48ΔC- /Tm3ser | This study | N/A |
| PTB-EGFP trap : w-;Bl/CyO;GFP-PTB/TM6b | Besse et al. (2009) | N/A |
| Bruno-EGFP trap : w-; GFP-Bruno/CyO | BDSC | BDSC Cat# 60144; RRID:BDSC_51324 |
| pHsp83-MCP-2xEGFP/CyO | This study | N/A |
| pHsp83-MCP-EGFP-FUS LC/CyO | This study | N/A |
| If/CyO; pHsp83-MCP-EGFP-FUS LC 12E/Tm3ser | This study | N/A |
| oskarGAL4/Tm3sb | BDSC | BDSC Cat# 44242; RRID:BDSC_51324 |
| oskarA87 | Jenny et al. (2006) | N/A |
| oskarattP,3P3GFP | Gáspár et al., (2017a) | N/A |
| oskar84 | Lehmann and Nusslein-Volhard (1986) | N/A |
| Df3RpXT103 | Lehmann and Nusslein-Volhard (1986) | N/A |
| Software | ||
| Fiji | Schindelin et al. (2012) | https://fiji.sc |
| xsPT Fiji plugin | Gaspar and Ephrussi (2017) | https://github.com/Xaft/xs/blob/master/_xs.jar |
| Cort Analysis Fiji plugin | Gaspar et al. (2014) | N/A |
| Imaris | Bitplane | https://imaris.oxinst.com |
| Huygens Essential | Scientific Volume Imaging, Hilversum, the Netherlands | https://svi.nl/Huygens-Deconvolution |
| FRAP Analyser | Halavatyi and Terjung (2017) | https://github.com/ssgpers/FRAPAnalalyser |
| PLAAC | Lancaster et al. (2014) | http://plaac.wi.mit.edu/ |
| IUPred | Mészáros et al., (2018) | https://iupred2a.elte.hu |
| IMOD v.4.9 | Kremer et al. (1996) | https://bio3d.colorado.edu/imod/ |
| TOM package | Nickell et al. (2005) | MATLAB (MathWorks) |
| SerialEM v3.7.2 | Mastronarde (2005) | https://bio3d.colorado.edu/SerialEM/ |
| WARP | Tegunov and Cramer (2019) | http://www.warpem.com/warp/ |
| Prism 8.0 | GraphPad | https://www.graphpad.com/ |
| JalView 2.11.1.3 | Waterhouse et al. (2009) | http://www.jalview.org |
| BioRender.com | Graphical Abstract created with BioRender.com | https://biorender.com |
∗
td: tandem, BDSC: Bloomington Drosophila Stock Center, DSHB: Developmental Studies Hybridoma Bank.