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. 2022 Apr 26;13:2245. doi: 10.1038/s41467-022-29969-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a The emission spectra for monitoring O₂^•− generation by Os2 (10 μM) using DHR123 probe (10 μM, λ_ex = 488 nm) in normoxia. b The ESR signal of O₂^•− trapped by DMPO (18 mM) after light irradiation in normoxia. c The ESR signal of ¹O₂ trapped by TEMP (8 mM) after light irradiation in normoxia. d The UV–Vis absorption spectra of ABDA (100 μM) for monitoring of ¹O₂ generated by Os2 (20 μM) under light irradiation in normoxia. e The plot of A₀-A of ABDA at 378 nm versus irradiation time in the presence of Os1 or Os2 in hypoxia or normoxia (n = 3 independent experiments). Error bars represent S.D. from the mean. f Schematic diagram of generating O₂^•−, ¹O₂ and OH• by light-activating Os2. Light irradiation: 465 nm, 13 mW/cm²; Experiment temperature: 298 K; SOD superoxide dismutase.