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. 2022 Mar 15;25(4):103964. doi: 10.1016/j.isci.2022.103964

Figure 1.

Figure 1

DEC2-P384R;AD and Npsr1-Y206H;AD mice have less tangles and plaques

(A) Immunostaining of PHFs tau located within the DG region of six-month-old PS19 mice (left), DEC2-WT;PS19 mice (middle) and DEC2-P384R;PS19 mice (right). PS19, n = 16; DEC2-WT;PS19, n = 10; DEC2-P384R;PS19, n = 17.

(B) Quantification of (A).

(C) Representative silver-stained slices for six-month-old female DEC2-WT or DEC2-P384R mice in 5XFAD background; female 5XFAD and Npsr1+/m;5XFAD mice.

(D) % area of silver-stained brain slice from hippocampus and cortex covered by plaques in six-month-old mice. Male hippocampus: DEC2-WT;5XFAD, n = 4 (N = 35 slices); DEC2-P384R;5XFAD, n = 6 (N = 49 slices); 5XFAD, n = 4 (N = 30 slices); Npsr1+/m;5XFAD, n = 8 (N = 58 slices). Female hippocampus: DEC2-WT;5XFAD, n = 3 (N = 21 slices); DEC2-P384R;5XFAD, n = 3 (N = 21 slices); 5XFAD, n = 5 (N = 35 slices); Npsr1+/m;5XFAD, n = 7 (N = 48 slices). Male cortex: DEC2-WT;5XFAD, n = 4 (N = 42 slices); DEC2-P384R;5XFAD, n = 7 (N = 61 slices); 5XFAD, n = 4 (N = 41 slices); Npsr1+/m;5XFAD, n = 8 (N = 68 slices). Female cortex: DEC2-WT;5XFAD, n = 3 (N = 21 slices); DEC2-P384R;5XFAD, n = 3 (N = 21 slices); 5XFAD, n = 4 (N = 35 slices); Npsr1+/m;5XFAD, n = 7 (N = 48 slices).

(E) Immunostaining of PHFs tau located within the DG region of six-month-old mice from left to right: PS19, n = 12; Npsr1+/m;PS19, n = 13; Npsr1+/m, n = 2; WT, n = 2.

(F) Quantification of (E). One-way ANOVA with Fisher’s LSD test (B). One-way ANOVA with post hoc Tukey’s multiple comparisons test (D). Two-way ANOVA with post hoc Tukey’s multiple comparisons test (F).

Data expressed as mean ± SEM. ∗p < 0.05, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001; n.s., not significant. Scale bars, 50 μm (A and E) and 1mm (C).