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. 2022 Apr 4;25(4):104089. doi: 10.1016/j.isci.2022.104089

Figure 2.

Figure 2

The phosphorylation level of p38 is increased in the neurosphere derived from the hiPSC (201B7) overexpressing GADD45G

(A) Quantification of the expression level of phosphorylated p38 when GADD45G is overexpressed in the hiPSC (201B7)-derived neurospheres with a lentiviral vector (n = 3, p = 0.000936).

(B) Immunostaining images of phosphorylated p38 in the neurosphere derived from the hiPSC (201B7) overexpressing GADD45G. Venus is used as a reporter in the control lentiviral vector and EGFP is used as a reporter in the GADD45G overexpressing lentiviral vector.

(C) Quantification of the expression level of total p38 when GADD45G is overexpressed in the hiPSC (201B7)-derived neurospheres with a lentiviral vector (n = 3, p = 0.107).

(D) Immunostaining images of total p38 in the neurosphere derived from the hiPSC (201B7) overexpressing GADD45G. Venus is used as a reporter in the control lentiviral vector and EGFP is used as a reporter in the GADD45G overexpressing lentiviral vector.Statistical analyses were performed with unpaired two-tailed Student’s t-tests. The values in the bar graphs represent the means ± SEs. 10 neurospheres were observed for each experiment. ∗∗∗p < 0.001. Scale bars: 100 μm.