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. 2022 Apr 4;25(4):104089. doi: 10.1016/j.isci.2022.104089

Figure 5.

Figure 5

Increased phospho-p38/phospho-CDC25B expression after neuronal differentiation further increases neurite length

(A and B) On day 5 after the induction of neuronal differentiation from hiPSC-NS/PCs, DMSO (A) or RK-682 (B) was added, and neurons were cultured for 48 h. Representative images obtained by the time-lapse method are shown. Scale bars: 50 μm.

(C) Magnified images of neurites elongated by RK-682. Scale bars: 50 μm.

(D) RK-682 was added to the neurons, and the degree of neurite outgrowth was measured and quantified 2 days later. All neurons in four visual fields were quantified for each experiment (n = 3; p = 0.0494).Statistical analyses were performed with unpaired two-tailed Student’s t-tests. The values in the bar graphs represent the means ± SEs. ∗p < 0.05.