Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 26;13:2240. doi: 10.1038/s41467-022-29718-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a Table listing the naturally presented epitopes used in our studies and their microbial pathogen origin. b Schematic of experimental design for experiments in C, D. c WT/Il2ra^mut/mut or WT/Il2ra^mut/mut/Il2ra^−/− mixed BM chimeras (ratio 1:1:1) were infected i.v. with indicated pathogens 6–8 weeks post-reconstitution, and challenged with the same autologous pathogen 4–5 weeks after before staining for FACS analysis 6.5 days later using indicated MHC class I tetramers (Tet⁺) and appropriate congenic markers. Bar graphs show the relative fold expansion of Tet⁺ CD8⁺ T cells across 2 replicate experiments (n = 8–15 mice). d WT/Il2ra^mut/mut mixed BM chimeras were set up as in (B), infected and then challenged 4 weeks later with indicated pathogens before staining spleen cells for FACS analysis 4 or 6.5 days later. Bar graphs show the relative fold expansion of Tet⁺ CD8⁺ T cells across 2 replicate experiments (n = 5–12 mice). e Il2ra^mut/mut and WT P14 cells were adoptively transferred to naive hosts, subsequently infected the next day with Lm-GP_33–41. Il2ra^mut/mut and WT P14 memory cells were sorted 4 weeks later, transferred (ratio 1:1) to naive hosts, and challenged the next day with Lm-GP_33-41. Spleens cells were stained for FACS analysis 6.5 days later. Bar graph shows the relative frequency of Il2ra^mut/mut and WT P14 memory cells across 3 replicate experiments (n = 15 mice, p < 0.00001). Each symbol (c–e) represents one mouse and p-values are indicated when relevant with *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant, using two-tailed paired Student’s t test. f H2-K^b and H2-D^b RMA-S stabilization assay by indicated peptides. Data are presented as average MFI values and error bars indicate SEM. Kd values represent the peptide concentration required to achieve half of maximum cell-surface K^b or D^b expression. Data represent 1 of 3 independent replicate experiments. P-value is calculated using two-tailed unpaired Student’s t test on Kb MFI at 120 nM of peptide (p = 0.005287).